The Treetops Hotel in the Aberdare national park became known for its royal connection after George VI died while his daughter, then Princess Elizabeth, was staying there in 1952.
A beacon will be lit at the hotel on June 4 as part of a network of Diamond Jubilee Beacons across the UK, the Channel Islands, Isle of Man, Commonwealth and overseas territories.
The Queen will light the National Beacon on the Mall in London.
Pageant-master Bruno Peek said: "I am delighted that the government of Kenya has agreed to light a beacon in such an historic location as part of this unique event and I applaud them for their initiative."
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